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Monkeypox Virus PCR Rapid Test Kit Real Time For Human Serum Lesion Exudate Samples

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Monkeypox Virus PCR Rapid Test Kit Real Time For Human Serum Lesion Exudate Samples

Monkeypox Virus PCR Rapid Test Kit Real Time For Human Serum Lesion Exudate Samples
Monkeypox Virus PCR Rapid Test Kit Real Time For Human Serum Lesion Exudate Samples Monkeypox Virus PCR Rapid Test Kit Real Time For Human Serum Lesion Exudate Samples

Large Image :  Monkeypox Virus PCR Rapid Test Kit Real Time For Human Serum Lesion Exudate Samples

Product Details:
Place of Origin: Shenzhen, China
Brand Name: LABNOVATION
Payment & Shipping Terms:
Minimum Order Quantity: 10000 Tests
Price: Negotiation
Packaging Details: 24 Tests/Kit,48 Tests/Kit,96 Tests/Kit
Delivery Time: 7-10 working days
Supply Ability: 500000 Tests/Day

Monkeypox Virus PCR Rapid Test Kit Real Time For Human Serum Lesion Exudate Samples

Description
Product Name: Monkeypox Virus Real Time PCR Rapid Test Kit Ct Value: 35
Storage: -20℃±5℃ Detection Channels: FAM
IC: VIC LOD: 200 Copies/mL
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Monkeypox Virus PCR Rapid Test Kit

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Monkeypox PCR Rapid Test Kit

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Serum Monkeypox Real Time PCR Kit

Monkeypox Virus Real Time PCR Rapid Test Kit​

Intended Use


This kit is used for in vitro qualitative nucleic acid detection of Monkeypox Virus in Human serum, lesion exudate samples and swab specimens. The test results of this kit are for clinical reference only and should not be used as the only standard for clinical diagnosis.
Monkeypox is a rare zoonotic disease characterized by skin eruptions caused by the Monkeypox virus (MPV) and whose clinical symptoms are similar to smallpox. Transmitted mainly through animals, humans can contract monkeypox from bites from infected animals or from direct contact with the blood, fluids and rashes of infected animals, and the virus can also be transmitted from person to person.

 

Specification

 

Product Name Monkeypox Virus Real Time PCR Kit
Detection Channels FAM
IC VIC
Ct Value 35
Limit of Detection 200 Copies/mL
Storage -20℃±5℃
Transportation Under frozen condition
Shelf Life 12 Months
Sample Type Nasal swabs, oropharyngeal swab, saliva, urine, lesion tissue, exudate, and blood, etc.
Package 24 Tests/Kit,48 Tests/Kit,96 Tests/Kit
Components PCR Reaction Buffer, PCR Enzyme Mixture, Positive control, Negative control

 

 


TEST PRINCIPLE

 

This kit adopts real time fluorescence PCR technology, which is suitable for nucleic acid detection of monkeypox virus extracted from clinical samples such as Human serum, lesion exudate samples and swab specimens Each reaction system contains specific primers and fluorescent probes to detect the virus, and the monkeypox virus nucleic acid can be qualitatively detected by collecting the fluorescent signal generated by PCR amplification.
In addition, specific primers and fluorescent probes for the internal standard control for human clinical specimen detection are added to each reaction system, and the collection, transportation and extraction process of the sample to be measured is monitored, indicating the false negative of the test result.

 


APPLICABLE INSTRUMENT

 

Real-Time PCR System: Molarray MA-6000, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM 5,Hongshi SLAN-96S/96P, AGS8830, AGS4800.

 

COMPONENTS

 
 
Specifications LQ-000301
24T
LQ-000302
48T
LQ-000303
96T
PCR Reaction Buffer 336μL×1 tube 672μL×1 tube 672μL×2 tube
PCR Enzyme Mixture 30μL×1 tube 50μL×1 tube 100μL×1 tube
Positive control 50μL×1 tube 100μL×1 tube 200μL×1 tube
Negative control 50μL×1 tube 100μL×1 tube 200μL×1 tube
Instruction for use (pcs) 1 1 1

 

 

Monkeypox Virus PCR Rapid Test Kit Real Time For Human Serum Lesion Exudate Samples 0


PROCEDURES

1. Sample preparations
DNA extraction from clinical samples is conducted according to the corresponding requirements and procedures in the virus DNA extraction kit. The extracted

DNA can be directly used for detection. If the sample is not detected immediately after extraction, it should be stored at -70°C for standby, avoiding repeated freeze-thaw cycles.


2. Reaction system preparation
(1) System preparation: Take out the reagent from the kit, and allow it to thaw completely at room temperature. Invert the mixture and centrifuge immediately. N test reactions (N = number of samples to be tested + positive control + negative control + 1) are prepared for reaction systems, respectively, as follows.

Components Volume for 1 reaction system Volume for N reaction system
PCR reaction buffer 14μL 14μLx N
PCR Enzyme mixture 1μL 1μLx N
Total volume 15μL 15μLx N

(2) Reaction system distribution: Mix well the above reaction buffer, centrifuge, and dispense 15μL of aliquots into PCR tubes suitable for fluorescent PCR instrument. (Centrifuge the PCR tubes at 6,000rpm for 30s and transport to sample treatment zone.)


3. Sample loading (Sample treatment zone)
Add 10μL nucleic acid sample, negative control and positive control to the above PCR tubes respectively. Cap the tubes tightly and centrifuge at 6,000rpm for 10s and then transport to PCR amplification zone.
* Precaution: Adding samples in the following order is
recommended: negative control-> nucleic acid sample -> positive control.
 

4. PCR amplification (PCR amplification zone)
4.1 Put the caped PCR tubes into real-time PCR machine for amplification.
4.2 Fluorescence PCR cycle condition setting

Program Temperature Reaction duration Number of cycles

1

50°C 2 min 1
2 95°C 2s 1
3 95°C 1s 41
60°C 13s/20s/35s

Collect fluorescent signals at step 3:60℃; 35s for ABI 7500, 20s for SLAN-96S/96P, while 13s for other Real-Time PCR Systems. The total volume:25μL.

4.3 Disposal after detection
Dispose the PCR tubes in a sealed bag after reaction and treat the used tubes as medical wastes.
 

5. Settings for result analysis
Set the baseline at a region before the exponential amplification where the fluorescent signals of all the samples are relatively stable (no significant fluctuations in all the samples); set the starting point (cycle number) away from the signal fluctuations at the starting
phase of fluorescence collection; set the end point (cycle number)1~3 cycles before the Ct of the first sample to enter exponential amplification. 4~15 cycles are recommended.
Set the threshold right above the highest point of the negative control amplification curve (irregular noise curve).
 

6. Quality control criteria
Prior to evaluating the specimen results, the Positive Control and Negative Control should be interpreted using the interpretation table below, and the Positive Control and Negative Control curve must be performed correctly, otherwise the sample result is invalid.

Channels/
Controls
Cycle threshold(Ct)value
FAM VIC
Positive control Ct > 40 or UNDET Ct > 40 or UNDET
Negative control Ct ≤ 35 Ct ≤ 35

 

7. Interpretation of Test Results
FAM channels for Monkeypox Virus, detection result should be interpreted as below.
a) Positive: Ct ≤ 38 and amplification curve is S-shaped.
b) Suspected: 38 < Ct ≤ 40 and amplification curve is S-shaped, a second test is needed. Consider positive if Ct ≤ 40 and amplification curve is S-shaped for the second test. Considered negative if Ct > 40 or null Ct and Ct ≤ 35 in VIC channel for the second test.
c) Negative: Ct > 40 or null Ct and Ct ≤ 35 in VIC channel.
d) Re-test: Ct > 40 or null Ct and Ct > 35 in VIC channel.

 

Contact Details
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Contact Person: Ld

Tel: +8613480985156

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